![Western Blot: WDR5 Antibody [NBP1-30070]](https://resources.rndsystems.com/images/products/WDR5-Antibody-Western-Blot-NBP1-30070-img0003.jpg "Western Blot: WDR5 Antibody [NBP1-30070]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Loading...
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western, Immunoprecipitation (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Loading...
Product Specifications
Immunogen
maps to a region between residue 50 and 100 of human WD repeat domain 5 using the numbering given in entry NP_060058.1
Reactivity Notes
D. melanogaster, X. tropicalis (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for WDR5 Antibody
Western Blot: WDR5 Antibody [NBP1-30070]
Western Blot: WDR5 Antibody [NBP1-30070] - Detection of mouse and rat WDR5 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody NBP1-30070 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070]](https://resources.rndsystems.com/images/products/WDR5-Antibody-Immunohistochemistry-NBP1-30070-img0002.jpg "Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070]")
Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070]
Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- WDR5 used at a dilution of 1:200 (1ug/ml). Detection: DAB![Western Blot: WDR5 Antibody [NBP1-30070]](https://resources.rndsystems.com/images/products/WDR5-Antibody-Western-Blot-NBP1-30070-img0001.jpg "Western Blot: WDR5 Antibody [NBP1-30070]")
Western Blot: WDR5 Antibody [NBP1-30070]
Western Blot: WDR5 Antibody [NBP1-30070] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. WDR5 was successfully IPed by NBP1-30063.Applications for WDR5 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:2000-1:10000
Application Notes
IP Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
See Simple Western Antibody Database for Simple Western validation: tested in Human Cancer Cell Line; antibody dilution of 1:10; separated by size; detects a band at 47 kDa
See Simple Western Antibody Database for Simple Western validation: tested in Human Cancer Cell Line; antibody dilution of 1:10; separated by size; detects a band at 47 kDa
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: WDR5
Long Name
WD Repeat-containing Protein 5
Alternate Names
BIG3, SWD3
Gene Symbol
WDR5
Additional WDR5 Products
Product Documents for WDR5 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for WDR5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for WDR5 Antibody
There are currently no reviews for this product. Be the first to review WDR5 Antibody and earn rewards!
Have you used WDR5 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...