WDR77 Antibody (8B2F7) - BSA Free

Novus Biologicals | Catalog # NBP3-27910

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 8B2F7

Format

BSA Free
View all WDR77 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human WDR77

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for WDR77 Antibody (8B2F7) - BSA Free

WDR77 Antibody (8B2F7)

Western Blot: WDR77 Antibody (8B2F7) [NBP3-27910] -

Western Blot: WDR77 Antibody (8B2F7) [NBP3-27910] - Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for WDR77 Antibody (8B2F7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WDR77

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Alternate Names

Androgen receptor cofactor p44, MEP-50, MEP50Nbla10071, methylosome protein 50, MGC2722, p44, p44/Mep50, RP11-552M11.3, WD repeat domain 77, WD repeat-containing protein 77

Gene Symbol

WDR77

UniProt

Q9BQA1 (Human)

Additional WDR77 Products

Product Documents for WDR77 Antibody (8B2F7) - BSA Free

Certificate of Analysis

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Product Specific Notices for WDR77 Antibody (8B2F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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