WFS1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94845

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WFS1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human WFS1 (NP_005996.2). MDSNTAPLGPSCPQPPPAPQPQARSRLNATASLEQERSERPRAPGPQAGPGPGVRDAAAPAEPQAQHTRSRERADGTGPTKGDMEIPFEEVLERAKAGDPKAQTEVGKHYLQLAGDTDEELNSCTAVDWLVLAAKQGRREAVKLLRRCLADRRGITSENEREVRQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVAELLENVGQVNEHDGGAQPGPVPKSLQKQRRMLERLVSSESKNYIALDDFVEITKKYAKGVIPSSLFLQDDEDDDELAGKSPEDLPLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WFS1 Antibody - BSA Free

WFS1 Antibody - BSA Free

Western Blot: WFS1 Antibody - BSA Free [NBP2-94845] -

Western Blot: WFS1 Antibody - BSA Free [NBP2-94845] - Western blot analysis of various lysates, using WFS1 antibody (A1705) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
WFS1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: WFS1 Antibody - BSA Free [NBP2-94845] -

Immunocytochemistry/ Immunofluorescence: WFS1 Antibody - BSA Free [NBP2-94845] - Immunofluorescence analysis of HeLa cells using WFS1 Rabbit pAb (A1705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for WFS1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WFS1

Wolframin (WFS1) is a multipass endoplasmic reticulum (ER) membrane protein that plays an important role in maintaining homeostasis of the ER in the pancreas. WFS1 helps to regulate cellular calcium cation homeostasis by altering the filling state of the ER calcium cation store.

WFS1 is the major component of Wolfram syndrome, a rare form of juvenile diabetes, also known as diabetes insipidus and mellitus with optic atrophy and deafness syndrome (DIDMOAD). WFS1 is normally up-regulated during insulin secretion, and inactivation of the protein causes ER stress. In turn, chronic ER stress is thought to play a major role in Wolfram syndrome.

Long Name

Wolfram Syndrome 1

Alternate Names

DFNA14, DFNA38, DFNA6, DIDMOAD, WFRS, Wolframin

Gene Symbol

WFS1

Additional WFS1 Products

Product Documents for WFS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WFS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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