WIPF1/WIP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86859

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WIPF1/WIP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WIPF1/WIP Antibody - BSA Free

WIPF1/WIP Antibody - BSA Free Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
WIPF1/WIP Antibody - BSA Free Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
WIPF1/WIP Antibody - BSA Free Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Staining of human lung shows moderate cytoplasmic positivity in macrophages.
WIPF1/WIP Antibody - BSA Free Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Immunohistochemistry-Paraffin: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Staining of human testis shows no cytoplasmic positivity in cells in seminiferous ducts as expected.
WIPF1/WIP Antibody - BSA Free Western Blot: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Western Blot: WIPF1/WIP Antibody - BSA Free [NBP1-86859]

Analysis in human cell line HEL.

Applications for WIPF1/WIP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WIPF1/WIP

WIPF1 is involved in the organization of the actin cytoskeleton. The protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these WIPF1 and Wiskott-Aldrich syndrome protein may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. WIPF1 binds to Wiskott-Aldrich syndrome protein, profilin and actin. It is involved with NCK1 and GRB2 in the recruitment and activation of N-WASP and may be involved in regulating the subcellular localization of N-WASP, resulting in the disassembly of stress fibers in filopodia formation.

Alternate Names

WAS/WASL interacting protein family, member 1, WAS/WASL-interacting protein family member 1, WASP-interacting protein, WASPIP, WIPMGC111041, WiskProtein PRPL-2, WiskPRPL-2

Gene Symbol

WIPF1

Additional WIPF1/WIP Products

Product Documents for WIPF1/WIP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WIPF1/WIP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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