Wnt-8b Antibody (HL1549) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25756
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1549 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Wnt-8b. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (81%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Wnt-8b Antibody (HL1549) - Azide and BSA Free
Western Blot: Wnt-8b Antibody (HL1549) - Azide and BSA Free [NBP3-25756] -
Western Blot: Wnt-8b Antibody (HL1549) - Azide and BSA Free [NBP3-25756] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8b antibody [HL1549] (NBP3-25756) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Wnt-8b Antibody (HL1549) - Azide and BSA Free [NBP3-25756] -
Western Blot: Wnt-8b Antibody (HL1549) - Azide and BSA Free [NBP3-25756] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8b antibody [HL1549] (NBP3-25756) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Wnt-8b Antibody (HL1549) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Wnt-8b
Long Name
Wingless-type MMTV Integration Site Family, Member 8b
Alternate Names
Wnt8b
Gene Symbol
WNT8B
Additional Wnt-8b Products
Product Documents for Wnt-8b Antibody (HL1549) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Wnt-8b Antibody (HL1549) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling
