XLF Antibody

Novus Biologicals | Catalog # NB100-2258

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all XLF Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 250 and the C-terminus (residue 299) of human XRCC4-Like Factor using the numbering given in entry NP_079058.1 (GeneID 79840).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XLF Antibody

Western Blot: XLF Antibody [NB100-2258]

Western Blot: XLF Antibody [NB100-2258]

Western Blot: XLF Antibody [NB100-2258] - Detection of Human XLF on HeLa whole cell lysate using NB100-2258. XLF was also immunoprecipitated using rabbit anti-XLF antibody NB100-2257.
Immunohistochemistry-Paraffin: XLF Antibody [NB100-2258]

Immunohistochemistry-Paraffin: XLF Antibody [NB100-2258]

Immunohistochemistry-Paraffin: XLF Antibody [NB100-2258] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XLF used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for XLF Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2 -5 ug/mg lysate

Western Blot

1:2000 -1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XLF

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Alternate Names

Cernunnos, non-homologous end-joining factor 1, nonhomologous end-joining factor 1, Protein cernunnos, XLFFLJ12610, XRCC4-like factor

Entrez Gene IDs

79840 (Human)

Gene Symbol

NHEJ1

UniProt

NP_079058.1 (Human)

Additional XLF Products

Product Documents for XLF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for XLF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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