XPE/DDB2 Antibody (23GB2805)

Novus Biologicals | Catalog # NBP3-51044

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2805
View all XPE/DDB2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human XPE/DDB2 (Uniprot: Q92466)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XPE/DDB2 Antibody (23GB2805)

XPE/DDB2 Antibody (23GB2805)

Immunocytochemistry/ Immunofluorescence: XPE/DDB2 Antibody (23GB2805)[NBP3-51044] -

XPE/DDB2 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: DDB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
XPE/DDB2 Antibody (23GB2805)

Immunocytochemistry/ Immunofluorescence: XPE/DDB2 Antibody (23GB2805)[NBP3-51044] -

Immunocytochemical staining of HT-1080 cells with XPE/DDB2 antibody (1:1,000). Nuclei were stained blue with DAPI; XPE/DDB2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
XPE/DDB2 Antibody (23GB2805)

Flow Cytometry: XPE/DDB2 Antibody (23GB2805)[NBP3-51044] -

Validation of XPE/DDB2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with XPE/DDB2 antibody (1:2,000) and analyzed using BD flow cytometer.
XPE/DDB2 Antibody (23GB2805)

Flow Cytometry: XPE/DDB2 Antibody (23GB2805)[NBP3-51044] -

Flow cytometric analysis of XPE/DDB2expression in HT-1080 cells using XPE/DDB2 antibody (1:2,000). Green, isotype control; red, DDB2.
XPE/DDB2 Antibody (23GB2805)

Western Blot: XPE/DDB2 Antibody (23GB2805)[NBP3-51044] -

Western blotting analysis using XPE/DDB2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
XPE/DDB2 Antibody (23GB2805)

Western Blot: XPE/DDB2 Antibody (23GB2805)[NBP3-51044] -

Western blotting analysis using XPE/DDB2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit (damage specific DNA binding protein 2.

Applications for XPE/DDB2 Antibody (23GB2805)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XPE/DDB2

DDB2, also known as DNA damage-binding protein 2, consists of five isoforms of sizes 47.9 kDa, 26.7 kDa, 17.4 kDa, 40.7 kDa, and 26.8 kDa and is involved in repairing DNA damaged by ultraviolet light. Current research is being conducted on disorders and diseases including immunodeficiency, hepatitis, multiple sclerosis, lung cancer, ovarian cancer, skin cancer, and autosomal recessive disease. The protein has been linked to DNA repair and protein stability pathways where it interacts with CUL4A, DDB1, COPS5, ABL1, and E2F1.

Long Name

Xeroderma Pigmentosum E

Alternate Names

DDB2, XP5

Gene Symbol

DDB2

Additional XPE/DDB2 Products

Product Documents for XPE/DDB2 Antibody (23GB2805)

Certificate of Analysis

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Product Specific Notices for XPE/DDB2 Antibody (23GB2805)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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