XPV/DNA polymerase eta Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87166

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all XPV Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEETVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XPV/DNA polymerase eta Antibody - BSA Free

Western Blot: XPV/DNA polymerase eta Antibody [NBP1-87166]

Western Blot: XPV/DNA polymerase eta Antibody [NBP1-87166]

Western Blot: XPV/DNA polymerase eta Antibody [NBP1-87166] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: XPV/DNA polymerase eta Antibody [NBP1-87166]

Immunohistochemistry-Paraffin: XPV/DNA polymerase eta Antibody [NBP1-87166]

Immunohistochemistry-Paraffin: XPV/DNA polymerase eta Antibody [NBP1-87166] - Staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.

Applications for XPV/DNA polymerase eta Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XPV

DNA polymerase eta is specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. It also plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. It targets POLI to replication foci (referenced from swissprot).

Long Name

Xeroderma Pigmentosum Variant Type Protein

Alternate Names

DNA Polymerase, eta, POLH, RAD30A

Entrez Gene IDs

5429 (Human)

Gene Symbol

POLH

UniProt

Q9Y253

Additional XPV Products

Product Documents for XPV/DNA polymerase eta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for XPV/DNA polymerase eta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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