YAP1 Antibody (CL13628) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43868

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13628

Format

Azide and BSA Free
View all YAP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P46937, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for YAP1 Antibody (CL13628) - Azide and BSA Free

YAP1 Antibody (CL13628) - Azide and BSA Free Immunohistochemistry-Paraffin: YAP1 Antibody [NBP3-43868]

Immunohistochemistry-Paraffin: YAP1 Antibody [NBP3-43868]

Staining of human anaplastic oligodendroglioma shows strong cytoplasmic positivity in tumor cells.
YAP1 Antibody (CL13628) - Azide and BSA Free Immunohistochemistry-Paraffin: YAP1 Antibody [NBP3-43868]

Immunohistochemistry-Paraffin: YAP1 Antibody [NBP3-43868]

Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
YAP1 Antibody (CL13628) - Azide and BSA Free Immunohistochemistry-Paraffin: YAP1 Antibody [NBP3-43868]

Immunohistochemistry-Paraffin: YAP1 Antibody [NBP3-43868]

Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
YAP1 Antibody (CL13628) - Azide and BSA Free Immunohistochemistry-Paraffin: YAP1 Antibody [NBP3-43868]

Immunohistochemistry-Paraffin: YAP1 Antibody [NBP3-43868]

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Applications for YAP1 Antibody (CL13628) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: YAP1

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Long Name

Yes-associated Protein 1

Alternate Names

YAP2, YAP65, YKI, Yorkie Homolog

Gene Symbol

YAP1

Additional YAP1 Products

Product Documents for YAP1 Antibody (CL13628) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for YAP1 Antibody (CL13628) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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