YTHDC1 Antibody (3K8D1)

Novus Biologicals | Catalog # NBP3-33486

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 3K8D1
View all YTHDC1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 597-736 of mouse YTHDC1 (NP_808348.2).

Sequence:
SYNDYVREFHNMGPPPPWQGMPPYPGIEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERICDRDRDRGERGRYRR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for YTHDC1 Antibody (3K8D1)

YTHDC1 Antibody (3K8D1)

Western Blot: YTHDC1 Antibody (3K8D1) [NBP3-33486] -

Western Blot: YTHDC1 Antibody (3K8D1) [NBP3-33486] - Western Blot analysis of lysates from HeLa cells using [KO Validated] YTHDC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
YTHDC1 Antibody (3K8D1)

Western Blot: YTHDC1 Antibody (3K8D1) [NBP3-33486] -

Western Blot: YTHDC1 Antibody (3K8D1) [NBP3-33486] - Western Blot analysis of lysates from YTHDC1-cKO mouse ES cells, using [KO Validated] YTHDC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C. YTHDC1-cKO mouse ES cells were treated by 4OHT.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.

Applications for YTHDC1 Antibody (3K8D1)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: YTHDC1

YTHDC1 may be part of a signal transduction pathway that influences splice site selection

Long Name

YTH Domain Containing 1

Alternate Names

KIAA1966, YT521, YT521-B

Additional YTHDC1 Products

Product Documents for YTHDC1 Antibody (3K8D1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for YTHDC1 Antibody (3K8D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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