ZAK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33137

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZAK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZAK. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZAK Antibody - BSA Free

Western Blot: ZAK Antibody [NBP1-33137]

Western Blot: ZAK Antibody [NBP1-33137]

Western Blot: ZAK Antibody [NBP1-33137] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: ZAK Antibody [NBP1-33137]

Immunocytochemistry/ Immunofluorescence: ZAK Antibody [NBP1-33137]

Immunocytochemistry/Immunofluorescence: ZAK Antibody [NBP1-33137] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-33137]

Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-33137]

Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-33137] - Mahlarvu xenograft. ZAK antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for ZAK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZAK

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

AZK, EC 2.7.11, EC 2.7.11.25, HCCS-4, Human cervical cancer suppressor gene 4 protein, Leucine zipper- and sterile alpha motif-containing kinase, mitogen-activated protein kinase kinase kinase MLT, mixed lineage kinase 7, mixed lineage kinase with a leucine zipper and a sterile alpha motif, Mixed lineage kinase-related kinase, mixed lineage kinase-related kinase MRK-beta, MLK7, mlklak, MLK-like mitogen-activated protein triple kinase, MLK-related kinase, MLT, MLTK, MRKcervical cancer suppressor gene 4 protein, pk, protein kinase, sterile alpha motif and leucine zipper containing kinase AZK, Sterile alpha motif- and leucine zipper-containing kinase AZK

Gene Symbol

ZAK

Additional ZAK Products

Product Documents for ZAK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ZAK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ZAK Antibody - BSA Free

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  • Q: What is the difference between the two ZAK antibodies: NBP1-90367 and NBP1-33137? According to the amino acid sequence, the molecular weight of ZAK-alpha is about 91KDa and ZAK-beta is about 51KDa. NBP1-90367 corresponds to ZAK aa 157-305, which is the common amino acid sequence, so it can detect ZAK-alpha and ZAK-beta simultaneously. In your datasheet, the predicted band size is 51KDa. Additionally, in your or my western blot, I can't detect band in 91KDa. There is only one band in 51KDa. Is it reasonable that the band locating in 51kDa is ZAK-beta?

    A:

    Both of these antibodies were raised to the N-terminal region shared by both isoforms 1&2. NBP1-33137 was generated using a partial recombinant protein containing a sequence within aa 18-254 of the human form. NBP1-90367 was developed to partial recombinant protein corresponding to aa 157-305.
    Based on the immunogen sequence, both antibodies are predicted to recognize both isoform 1 and isoform 2.  
    There are two possible reasons why only one band shows on the WBs for each of these antibodies:
    1. In tested tissues only one isoform is expressed. According to uniprot (http://www.uniprot.org/uniprot/Q9NYL2#expression) Isoform 2 is the predominant form in all tissues, except for liver, in which isoform 1 is more highly expressed.
    2. These antibodies do indeed recognize only one isoform, despite shared immunogen sequence. We have not, however, tested these antibodies side by side on the same lysates, neither have we performed further specificity testing to rule out reactivity with the other isoform.
    Please also note that according to uniprot (http://www.uniprot.org/uniprot/Q9NYL2#sequences), there are actually 3 isoforms for MAP3K20, the third one is the shortest, yielding a protein of ~35kDa and NBP1-90367 is not going to recognize this isoform, but NBP1-33137 will.

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