ZC3H8 Antibody (OTI4H5) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-74908
![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0008.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4H5
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human ZC3H8 (NP_115883) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
33.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ZC3H8 Antibody (OTI4H5) - Azide and BSA Free
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0001.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0002.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0003.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0004.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0005.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0006.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0007.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]](https://resources.rndsystems.com/images/products/ZC3H8-Antibody-OTI4H5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74908-img0009.jpg "Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]")
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908]
Immunohistochemistry: ZC3H8 Antibody (OTI4H5) - Azide and BSA Free [NBP2-74908] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)Applications for ZC3H8 Antibody (OTI4H5) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: ZC3H8
Alternate Names
Fliz1, ZC3HDC8, zinc finger CCCH domain-containing protein 8, zinc finger CCCH type domain containing 8, zinc finger CCCH-type containing 8, zinc finger CCCH-type domain containing 8
Gene Symbol
ZC3H8
Additional ZC3H8 Products
Product Documents for ZC3H8 Antibody (OTI4H5) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZC3H8 Antibody (OTI4H5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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