ZEB1 Antibody (OTI3G6) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-74910
![Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-OTI3G6-Azide-and-BSA-Free-Western-Blot-NBP2-74910-img0002.jpg "Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]")
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3G6
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-350 of human ZEB1 (NP_110378) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This ZEB1 antibody does not cross-react with ZEB2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
123.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ZEB1 Antibody (OTI3G6) - Azide and BSA Free
Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]
Western Blot: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).![Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-OTI3G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74910-img0007.jpg "Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]")
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-OTI3G6-Azide-and-BSA-Free-Western-Blot-NBP2-74910-img0001.jpg "Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]")
Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]
Western Blot: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).![Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-OTI3G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74910-img0003.jpg "Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]")
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-OTI3G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74910-img0004.jpg "Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]")
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-OTI3G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74910-img0005.jpg "Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]")
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-OTI3G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74910-img0006.jpg "Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]")
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-OTI3G6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74910-img0008.jpg "Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]")
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Applications for ZEB1 Antibody (OTI3G6) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: ZEB1
Long Name
Zinc Finger E-box Binding Homeobox 1
Alternate Names
AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP
Gene Symbol
ZEB1
Additional ZEB1 Products
Product Documents for ZEB1 Antibody (OTI3G6) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZEB1 Antibody (OTI3G6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ZEB1 Antibody (OTI3G6) - Azide and BSA Free
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Q: In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
A: ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
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