![Western Blot: ZFP106 Antibody [NB100-74653]](https://resources.rndsystems.com/images/products/ZFP106-Antibody-Western-Blot-NB100-74653-img0006.jpg "Western Blot: ZFP106 Antibody [NB100-74653]")
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1300 and 1350 of human zinc finger protein 106 using the numbering given in entry NP_071918.1 (GeneID 64397).
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZFP106 Antibody
Western Blot: ZFP106 Antibody [NB100-74653]
Western Blot: ZFP106 Antibody [NB100-74653] - Detection of Human and Mouse ZFP106 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells, and whole cell lysate (50 ug) from Jurkat, Ramos, and Mouse CT26.WT cells. Antibody: Affinity purified rabbit anti-ZFP106 antibody NB100-74653 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.![Immunoprecipitation: ZFP106 Antibody [NB100-74653]](https://resources.rndsystems.com/images/products/ZFP106-Antibody-Immunoprecipitation-NB100-74653-img0007.jpg "Immunoprecipitation: ZFP106 Antibody [NB100-74653]")
Immunoprecipitation: ZFP106 Antibody [NB100-74653]
Immunoprecipitation: ZFP106 Antibody [NB100-74653] - Detection of human ZFP106 by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-ZFP106 antibody NB100-74653 used for IP at 6 ug per reaction. ZFP106 was also immunoprecipitated by rabbit anti-ZFP106 antibody NB100-74654. For blotting immunoprecipitated ZFP106, NB100-74653 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.Applications for ZFP106 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ZFP106
Alternate Names
DKFZp451A239, FLJ34610, SH3BP3, SH3-domain binding protein 3, zfp-106, zinc finger protein 106 homolog, zinc finger protein 106 homolog (mouse), Zinc finger protein 474, ZNF474FLJ45841
Entrez Gene IDs
64397 (Human)
Gene Symbol
ZFP106
UniProt
Additional ZFP106 Products
Product Documents for ZFP106 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZFP106 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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