![Immunohistochemistry-Paraffin: ZFP106 Antibody [NB100-74654]](https://resources.rndsystems.com/images/products/ZFP106-Antibody-Immunohistochemistry-Paraffin-NB100-74654-img0004.jpg "Immunohistochemistry-Paraffin: ZFP106 Antibody [NB100-74654]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1833 and 1883 of human zinc finger protein 106 using the numbering given in entry NP_071918.1 (GeneID 64397).
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZFP106 Antibody
Immunohistochemistry-Paraffin: ZFP106 Antibody [NB100-74654]
Immunohistochemistry-Paraffin: ZFP106 Antibody [NB100-74654] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- ZFP106 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: ZFP106 Antibody [NB100-74654]](https://resources.rndsystems.com/images/products/ZFP106-Antibody-Immunoprecipitation-NB100-74654-img0008.jpg "Immunoprecipitation: ZFP106 Antibody [NB100-74654]")
Immunoprecipitation: ZFP106 Antibody [NB100-74654]
Immunoprecipitation: ZFP106 Antibody [NB100-74654] - Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-ZFP106 antibody NB100-74654 (lot 2) used for IP at 6 ug per reaction. ZFP106 was also immunoprecipitated by rabbit anti-ZFP106 antibody NB100-74653. For blotting immunoprecipitated ZFP106, NB100-74654 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.Applications for ZFP106 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:250
Immunoprecipitation
2-5 mcg/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ZFP106
Alternate Names
DKFZp451A239, FLJ34610, SH3BP3, SH3-domain binding protein 3, zfp-106, zinc finger protein 106 homolog, zinc finger protein 106 homolog (mouse), Zinc finger protein 474, ZNF474FLJ45841
Entrez Gene IDs
64397 (Human)
Gene Symbol
ZFP106
UniProt
Additional ZFP106 Products
Product Documents for ZFP106 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZFP106 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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