ZNF135 Antibody - BSA Free

Western Blot: ZNF135 Antibody - BSA Free [NBP1-80913] -

PI3K/AKT and JNK mediate the effect of HNRNPA3 on SREBF1 activation. (A) Marc-145 cells were transfected with siNC or increasing amounts of siHNRNPA3 and infected with PEDV (MOI = 0.5) for 24 h. The protein levels of SREBF1, FASN, and ACC1 were determined by western blot. beta -Actin was used as a loading control. (B) Marc-145 cells were transfected with siHNRNPA3 and infected with PEDV (MOI = 0.5), then incubated with LY294002 (LY294, 10 uM), SP600125 (SP600, 10 uM), trametinib (Tram, 10 uM), or DMSO (1:1,000). The mRNA levels of SREBF1 were determined by qPCR. (C–E) Marc-145 cells were transfected with siHNRNPA3 and infected with PEDV (MOI = 0.5), then incubated with LY294002/SP600125/trametinib or DMSO. The protein levels of SREBF1 were determined by western blot. beta -Actin was used as a loading control. (F) Marc-145 cells were transfected and treated as described above. The cells were harvested for dual-luciferase assays. (G and H) Marc-145 cells were transfected and treated as described above. The mRNA levels of FASN and ACC1 were determined by qPCR, and the supernatant was harvested to assess the virus titer by TCID50. (I and J) Marc-145 cells were transfected and treated as described above. The mRNA and protein levels of ZNF135 were determined by qPCR and western blot. beta -actin was used as a loading control. Three independent experiments were carried out. Error bars represent the mean +/- SD for triplicate experiments. *P < 0.05, **P < 0.01. ns, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38259103), licensed under a CC-BY license. Not internally tested by Novus Biologicals.