ZNF354C Antibody - BSA Free

Immunohistochemistry-Paraffin: ZNF354C Antibody [NBP1-81352]

Immunohistochemistry-Paraffin: ZNF354C Antibody [NBP1-81352] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ZNF354C Antibody [NBP1-81352]

Immunohistochemistry-Paraffin: ZNF354C Antibody [NBP1-81352] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ZNF354C Antibody [NBP1-81352]

Immunohistochemistry-Paraffin: ZNF354C Antibody [NBP1-81352] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Chromatin Immunoprecipitation-exo-Seq: ZNF354C Antibody - BSA Free [NBP1-81352]

ChIP-Exo-Seq composite graph for Anti-ZNF354C (NBP1-81352) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western Blot: ZNF354C Antibody - BSA Free [NBP1-81352] -

The conserved amino acid sequence MLE within the KRAB domain of ZNF354C mediates transcriptional repression. (a) Schematic of the ZNF354C protein architecture with its KRAB domain and 11 C2H2 Zinc fingers. Amino acids within the KRAB domain as targets for site-directed mutagenesis are indicated. Numbers below the protein indicate the amino acid position. (b) Alignment of KRAB sequences of various ZNF proteins with ZNF354C as a reference. The numbers within the protein names indicate the amino acid positions within the respective protein. The underlined (blue) and red amino acids were subjected to site-directed mutagenesis. (c) Alignment of the human ZNF354C KRAB sequence with other mammalian ZNF354C homologues. The numbers within the protein names indicate the amino acid positions within the respective protein. The underlined (blue) and red amino acids were subjected to site-directed mutagenesis. (d, e) RT-qPCR (d) and western blot (e) of ZNF354C after overexpression of pCMV6-ZNF354C (354C) or the individual mutants DV (DV → AA), MLE (MLE → KKK) or EW (EW → AA). pCMV6-Entry served as negative control (CTL). Data in d is normalised to b-actin. n = 9. Data are mean +/- SEM. Paired t-test; *P < 0.05. In e, antibodies against ZNF354C and HSC70/HSP70 are shown. (f) RT-qPCR of RGS5, PATL2, HAP1, NRAV and HERC5 after overexpression of pCMV6-ZNF354C (WT, dotted line, set as 1) or the individual mutants DV (DV → AA), MLE (MLE → KKK) or EW (EW → AA). Data is normalised to b-actin. n = 3. Data are mean +/- SEM. Paired t-test; *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33154469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ZNF354C Antibody - BSA Free [NBP1-81352] -

Zinc Finger Protein 354C (ZNF354C) overexpression leads to inhibition of sprouting in HMEC-1. (a) RNA expression level as measured with RT-qPCR of ZNF354C in different cell types. Data is normalised to HUVEC batch 1 (set as 1) and to b-actin. n = 3. Data are mean +/- SEM. (b) Representative western blot of HUVEC, HMEC-1 and HAoSMC. ZNF354C antibody was used. HSC70/HSP70 antibodies served as loading control. (c) Immunofluorescence with an antibody against ZNF354C in HMEC-1, HUVEC and HAoSMC. Nuclei were stained with DAPI. Scale bar indicates 20 um. (d) RT-qPCR of ZNF354C after overexpression (OE) of pCMV6-ZNF354C (354C) in HMEC-1, HUVEC and HAoSMC. PCMV6-entry was used as negative control (CTL). Data is normalized to b-actin. n = 5. Data are mean +/- SEM. Mann–Whitney t-test; *P < 0.05. (e) Representative western blot of untreated (native) HMEC-1, HUVEC and HAoSMC or after overexpression of pCMV6-ZNF354C (ZNF354C) or pCMV6-entry (CTL). ZNF354C, Flag or b-actin antibodies were used. (f) Immunofluorescence with an antibody against ZNF354C in HMEC-1 after overexpression of pCMV6-ZNF354C or pCMV6-entry. Nuclei were stained with DAPI. Scale bar indicates 20 um. (g, h) Spheroid outgrowth assay quantification of sprout number (g) and cumulative sprout length (h) after overexpression of pCMV6-ZNF354C (354C) or pCMV6-entry (NC). Cells were studied under basal conditions or after treatment with VEGF-A or TNF-alpha. Scale bar indicates 50 um. n = 13. One-Way ANOVA with Bonferroni post-hoc test. *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33154469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: ZNF354C Antibody - BSA Free [NBP1-81352] -

Zinc Finger Protein 354C (ZNF354C) overexpression leads to inhibition of sprouting in HMEC-1. (a) RNA expression level as measured with RT-qPCR of ZNF354C in different cell types. Data is normalised to HUVEC batch 1 (set as 1) and to b-actin. n = 3. Data are mean +/- SEM. (b) Representative western blot of HUVEC, HMEC-1 and HAoSMC. ZNF354C antibody was used. HSC70/HSP70 antibodies served as loading control. (c) Immunofluorescence with an antibody against ZNF354C in HMEC-1, HUVEC and HAoSMC. Nuclei were stained with DAPI. Scale bar indicates 20 um. (d) RT-qPCR of ZNF354C after overexpression (OE) of pCMV6-ZNF354C (354C) in HMEC-1, HUVEC and HAoSMC. PCMV6-entry was used as negative control (CTL). Data is normalized to b-actin. n = 5. Data are mean +/- SEM. Mann–Whitney t-test; *P < 0.05. (e) Representative western blot of untreated (native) HMEC-1, HUVEC and HAoSMC or after overexpression of pCMV6-ZNF354C (ZNF354C) or pCMV6-entry (CTL). ZNF354C, Flag or b-actin antibodies were used. (f) Immunofluorescence with an antibody against ZNF354C in HMEC-1 after overexpression of pCMV6-ZNF354C or pCMV6-entry. Nuclei were stained with DAPI. Scale bar indicates 20 um. (g, h) Spheroid outgrowth assay quantification of sprout number (g) and cumulative sprout length (h) after overexpression of pCMV6-ZNF354C (354C) or pCMV6-entry (NC). Cells were studied under basal conditions or after treatment with VEGF-A or TNF-alpha. Scale bar indicates 50 um. n = 13. One-Way ANOVA with Bonferroni post-hoc test. *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33154469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.