ZNF827 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13599

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZNF827 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: LNVQFVSHMSLHVDKEQWMFSICCTACDFVTMEEAEIKTHIGTKHTGEDRKTPSESNSPSSSSLSALSDSANSKDDSDGSQKNKGGNNLLVISVMP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF827 Antibody - BSA Free

ZNF827 Antibody - BSA Free Immunohistochemistry: ZNF827 Antibody - BSA Free [NBP2-13599]

Immunohistochemistry: ZNF827 Antibody - BSA Free [NBP2-13599]

Staining of human testis shows moderate nuclear positivity in Leydig cells.
ZNF827 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: ZNF827 Antibody - BSA Free [NBP2-13599]

Immunocytochemistry/ Immunofluorescence: ZNF827 Antibody - BSA Free [NBP2-13599]

Staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
ZNF827 Antibody - BSA Free Immunohistochemistry: ZNF827 Antibody - BSA Free [NBP2-13599]

Immunohistochemistry: ZNF827 Antibody - BSA Free [NBP2-13599]

Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
ZNF827 Antibody - BSA Free Immunohistochemistry: ZNF827 Antibody - BSA Free [NBP2-13599]

Immunohistochemistry: ZNF827 Antibody - BSA Free [NBP2-13599]

Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.
ZNF827 Antibody - BSA Free Immunohistochemistry: ZNF827 Antibody - BSA Free [NBP2-13599]

Immunohistochemistry: ZNF827 Antibody - BSA Free [NBP2-13599]

Staining of human prostate shows strong nuclear positivity in glandular cells.
ZNF827 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ZNF827 Antibody - BSA Free [NBP2-13599]

Chromatin Immunoprecipitation-exo-Seq: ZNF827 Antibody - BSA Free [NBP2-13599]

ChIP-Exo-Seq composite graph for Anti-ZNF827 (NBP2-13599) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for ZNF827 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF827

ZNF827 may be involved in transcriptional regulation

Alternate Names

zinc finger protein 827

Gene Symbol

ZNF827

Additional ZNF827 Products

Product Documents for ZNF827 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ZNF827 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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