ZP2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-21053

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ZP2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZP2. The exact sequence is proprietary.

Localization

Processed zona pellucida sperm-binding protein 2: Secreted; extracellular space; extracellular matrix; Cell membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZP2 Antibody - Azide and BSA Free

Western Blot: ZP2 Antibody [NBP2-21053]

Western Blot: ZP2 Antibody [NBP2-21053]

Western Blot: ZP2 Antibody [NBP2-21053] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: ZP2 Antibody [NBP2-21053]

Immunohistochemistry-Paraffin: ZP2 Antibody [NBP2-21053]

Immunohistochemistry-Paraffin: ZP2 Antibody [NBP2-21053] - Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using antibody at 1:250 dilution.

Applications for ZP2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZP2

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. [provided by RefSeq]

Long Name

Zona Pellucida Glycoprotein 2 (Sperm Receptor)

Alternate Names

Zona Pellucida Protein A, Zp-2, ZPA

Gene Symbol

ZP2

UniProt

Q05996 (Human)

Additional ZP2 Products

Product Documents for ZP2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ZP2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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