Histone Deacetylases (HDACs) are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer.
Human histone Deacetylase 8 (HDAC8) is a member of the class I histone Deacetylases (HDACs). Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor trichostatin A.
Sirtuin 2, is a member of the class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3 - 4). The enzymatic activity of class III HDACs is nicotinamide adenine dinucleotide (NAD) dependent and insensitive to HDAC inhibitor trichostatin A