PerCP-conjugated Antibodies

Peridinin Chlorophyll Protein Complex (PerCP)-conjugated antibod­ies are convenient tools for use in flow cytometry experiments. PerCP is a water soluble carotenoid pigment found in photosynthetic dino­flagellates. It is excited by a 488 nm argon laser, and with a relatively large Stokes shift, emits at a maximum wavelength of 675 nm. Be­cause of these spectral characteristics, there is minimal overlap with other commonly used fluorochromes such as phycoerythrin (PE) or fluorescein. This makes PerCP-labeled antibodies especially useful for multi-color analysis with PE and fluorescein-conjugated antibodies. In addition, low cross talk between channels reduces the time spent setting fluorescent compensation.

Conjugated Antibody, CXCR4 Expression in Jurkat Cells Using Flow Cytometry.
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CXCR4 Expression in Jurkat Cells Using Flow Cytometry. Jurkat human leukemic T cells were labeled using PerCP-conjugated anti-human CXCR4 (Catalog # FAB170C; filled histogram) or with an isotype control antibody (Catalog # IC003C; open histogram).
Molecule Species Catalog #   Molecule Species Catalog #
Alkaline Phosphatase Human, Mouse, Rat FAB1448C   NKG2A Human FAB1059C
CCR2 Human FAB151C   NKG2C Human FAB138C
CCR3 Human, Mouse FAB155C, FAB729C   NKG2D Human FAB139C
CCR7 Human FAB197C   Oct-3/4 Human, Mouse IC1759C
CD3 epsilon Human FAB100C   SOX2 Human, Mouse IC2018C
CD4 Human FAB3791C   SSEA-4 Human, Mouse FAB1435C
CD8 alpha Human FAB1509C   T-bet/TBX21 Human IC5385C
CD19 Human FAB4867C   VEGF R2/KDR/Flk-1 Human FAB357C
CXCR2/IL-8 RB Human FAB331C   Goat F(ab)2 Anti-mouse IgG (H+L) Mouse F0114
CXCR3 Human, Mouse FAB160C, FAB1685C   Goat F(ab)2 Anti-rat IgG (H+L) Rat F0115
CXCR4 Human FAB170C   Mouse IgG1 PerCP Isotype Control Mouse IC002C
CXCR5 Human FAB190C   Mouse IgG2A PerCP Isotype Control Mouse IC003C
Endoglin/CD105 Human FAB10971C   Mouse IgG2B PerCP Isotype Control Mouse IC0041C
ErbB3/Her3 Human FAB3481C   Mouse IgG3 PerCP Isotype Control Mouse IC007C
IGF-I R Human FAB391C   Rat IgG2A PerCP Isotype Control Rat IC006C
IL-17/IL-17A Human IC3171C   Rat IgG2B PerCP Isotype Control Rat IC013C
LAP (TGF-beta 1) Human FAB2463C