Peridinin Chlorophyll Protein Complex (PerCP)-conjugated antibodies are convenient tools for use in flow cytometry experiments. PerCP is a water soluble carotenoid pigment found in photosynthetic dinoflagellates. It is excited by a 488 nm argon laser, and with a relatively large Stokes shift, emits at a maximum wavelength of 675 nm. Because of these spectral characteristics, there is minimal overlap with other commonly used fluorochromes such as phycoerythrin (PE) or fluorescein. This makes PerCP-labeled antibodies especially useful for multi-color analysis with PE and fluorescein-conjugated antibodies. In addition, low cross talk between channels reduces the time spent setting fluorescent compensation.
CXCR4 Expression in Jurkat Cells Using Flow Cytometry. Jurkat human leukemic T cells were labeled using PerCP-conjugated anti-human CXCR4 (Catalog # FAB170C; filled histogram) or with an isotype control antibody (Catalog # IC003C; open histogram).