Glutamatergic neurons produce the neurotransmitter glutamate, which is the main excitatory neurotransmitter in the mammalian central nervous system. It is involved in most of the brain’s fundamental processes such as cognition, learning, memory, and sensory perception. Dysregulation of glutamatergic neurotransmission is associated with many neurological disorders including epilepsy, schizophrenia, Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis, and stroke.
VGLUT1 in Human Brain.
The Vesicular Glutamate Transporter 1 (VGLUT1), also called SLC17A7, was detected in immersion-fixed paraffin-embedded sections of human brain using a Mouse Anti-Human VGLUT1/SLC17A7 Monoclonal Antibody (R&D Systems, Catalog # MAB9054
). The tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (R&D Systems, Catalog # CTS002
; brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic vesicles.
VGLUT2 in Human Brain.
The Vesicular Glutamate Transporter 2 (VGLUT2) was detected in immersion-fixed paraffin-embedded sections of human brain (cerebellum) using a Mouse Anti-Human/Mouse/Rat VGLUT2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-46641
). The cerebellar tissue was stained using HRP and DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glutamatergic synapses in the molecular and granular layers of the cerebellum.