Human VGLUT1/SLC17A7 Antibody

Catalog # Availability Size / Price Qty
MAB9054
MAB9054-SP
VGLUT1/SLC17A7  in Human Brain.
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Human VGLUT1/SLC17A7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VGLUT/SLC17A7 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 732607
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human VGLUT/SLC17A7
Asn93-Thr115
Accession # Q9P2U7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry VGLUT1/SLC17A7  in Human Brain. View Larger

VGLUT1/SLC17A7 in Human Brain. VGLUT1/SLC17A7 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human VGLUT1/SLC17A7 Monoclonal Antibody (Catalog # MAB9054) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic vesicles. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VGLUT1/SLC17A7

Vesicular glutamate transporter 1 (VGLUT1), also known as SLC17A7, is an integral membrane protein involved in glutamate uptake into synaptic vesicles. The vesicular glutamate transporter (VGLUT) mediates the accumulation of neurotransmitter (glutamate) into synaptic vesicles. It is expressed in pre-synaptic vesicles. VGLUT proteins appear to be differentially expressed in myelinated afferents located in specific axonal populations within the spinal cord. VGLUT proteins are among the most specific markers for the identification of glutamatergic neurons. It is an essential molecular component of glutamate synaptic transmission, and its de-regulation can result synaptic failure and excitotoxicity.

Long Name
Vesicular Glutamate Transporter 1
Entrez Gene IDs
57030 (Human); 72961 (Mouse); 116638 (Rat)
Alternate Names
BNPI; BNPIbrain-specific Na-dependent inorganic phosphate cotransporter; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; member 7; SLC17A7; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter); Solute carrier family 17 member 7; VGLUT1; VGLUT1vesicular glutamate transporter 1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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