Help & FAQs: Staining Kits and Reagents

• What coverslips and cell culture plates are recommended for ICC stainings on coverslips? 
• In house, we use 12 mm Cover Glasses from Carolina, Catalog # 633009. We use 24-well flat bottom tissue culture treated cell culture plates from Corning, Catalog # 3526.
• What is the concentration of the biotinylated secondary antibody in the Cell & Tissue Staining Kit?
• The concentraion of the secondary antibody is considered proprietary. The protocol instructs adding 1-3 drops of the secondary antibody directly to the tissue section. 

Cell and Tissue Staining Kits

• Are deparaffinization and antigen retrieval performed prior to starting IHC detection using Cell and Tissue Staining Kits (for example, Catalog # CTS019)?
• Yes, perform deparaffinization and, if necesary, antigen retrieval prior to treating samples with primary antibody, and then use the Cell and Tissue Staining Kit for detection.

Immunohistochemistry Reagents

• Are the Hydrophobic Coverslips, Catalog # 4867-100, reusable?
• We do not recommend reusing the coverslips.
• How many Hydrophobic Coverslips are provided in each package of Catalog # 4867-100?
• Each package contains 100 coverslips.
• What coverslips and cell culture plates are recommended for ICC stainings on coverslips? 
• In house, we use 12 mm Cover Glasses from Carolina, Catalog # 633009. We use 24-well flat bottom tissue culture treated cell culture plates from Corning, Catalog # 3526.
• What is the percent light transmission for the Hydrophobic Coverslips, Catalog # 4867-100?
• The coverslips transmit greater than 90% of visible light and are slightly opaque to UV light.