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Help & FAQs: Staining Kits and Reagents
- What coverslips and cell culture plates are recommended for ICC stainings on coverslips?
- In house, we use 12 mm Cover Glasses from Carolina, Catalog # 633009. We use 24-well flat bottom tissue culture treated cell culture plates from Corning, Catalog # 3526.
- What is the concentration of the biotinylated secondary antibody in the Cell & Tissue Staining Kit?
- The concentraion of the secondary antibody is considered proprietary. The protocol instructs adding 1-3 drops of the secondary antibody directly to the tissue section.
Cell and Tissue Staining Kits
- Are deparaffinization and antigen retrieval performed prior to starting IHC detection using Cell and Tissue Staining Kits (for example, Catalog # CTS019)?
- Yes, perform deparaffinization and, if necesary, antigen retrieval prior to treating samples with primary antibody, and then use the Cell and Tissue Staining Kit for detection.
Immunohistochemistry Reagents
- Are the Hydrophobic Coverslips, Catalog # 4867-100, reusable?
- We do not recommend reusing the coverslips.
- Can an R&D Systems antibody which has been validated for Immunocytochemistry (ICC or IF) be used in Immunohistochemistry (IHC)? Can an antibody which has been validated for IHC be used in ICC?
- R&D Systems will support an antibody that has been validated in-house for ICC or IHC in both applications. Although we cannot guarantee that an antibody will work in all cells or tissues, under all conditions, our validation shows that the antibody recognizes the fixed antigen. We may not have tested the antibody in both ICC and IHC, but R&D Systems will support its use in either application.
- How many Hydrophobic Coverslips are provided in each package of Catalog # 4867-100?
- Each package contains 100 coverslips.
- What coverslips and cell culture plates are recommended for ICC stainings on coverslips?
- In house, we use 12 mm Cover Glasses from Carolina, Catalog # 633009. We use 24-well flat bottom tissue culture treated cell culture plates from Corning, Catalog # 3526.
- What is the percent light transmission for the Hydrophobic Coverslips, Catalog # 4867-100?
- The coverslips transmit greater than 90% of visible light and are slightly opaque to UV light.