IGF-I in Human Placenta.
Insulin-like Growth Factor I (IGF-I) was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using Human IGF-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
This antibody specifically recognizes human IGF-I. In ELISA and Western blot experiments, this antibody shows approximately 35% cross-reactivity with recombinant mouse IGF-I.
Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
IGF‑I in MDA‑MB‑231 Human Cell Line.
Insulin-like Growth Factor I (IGF‑I) was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Human IGF‑I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IGF-I in Mouse Embryo.
Insulin-like Growth Factor I (IGF-I) was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.