Immunohistochemistry (IHC) and immunocytochemistry (ICC) are commonly used techniques employed to localize the cellular expression of antigens. This guide provides information from the scientists at R&D Systems IHC/ICC core facility, including:
- Techniques and suggestions for designing a successful IHC/ICC experiment.
- Protocols for chromogenic and fluorescent staining of antigens in tissue sections and cultured cells.
- A troubleshooting guide that will help solve a range of IHC/ICC-related issues.