|Polystyrene beads are read on a dual-laser flow-based detection instrument, such as the Luminex® 100™, Luminex 200™ or Bio-Rad® Bio-Plex® analyzer. One laser classifies the bead and determines the analyte that is being detected. The second laser determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound.
||In addition to the Luminex 100, Luminex 200 or Bio-Rad Bio-Plex dual-laser, flow-based analyzers, magnetic beads can be read using the Luminex MAGPIX® Analyzer. A magnet in the MAGPIX analyzer captures and holds the magnetic beads in a monolayer, while two spectrally distinct light-emitting diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and, the second LED determines the magnitude of the PE-derived signal. Each well is imaged with a CCD camera.