Home » Lessons from 20 Years in Antibody Validation and Reproducibility
Lessons from 20 Years in Antibody Validation and Reproducibility
Submitted by tim.philippi@bi... on Fri, 08/05/2016 - 15:35
Birte Aggeler, Ph.D., Director of Antibody Development and Flow Cytometry, Antibody Development Department
In this webinar you will learn more about the R&D Systems approach to developing antibodies, including the quality control specifications that we specifically designed to optimize the activity, reliability, and consistency of our antibodies and immunoassays. We will take you through the process from start to finish with case examples, highlighting our proper design and rigorous testing that allows us to address concerns on specificity and reproducibility. Antibody development is highly dependent on proper controls, sample preparation, and repeat testing. Other important factors in developing antibodies that address a specific problem are choosing the correct antibody type (mono- or polyclonal) and, in some cases, selecting the appropriate species of the host animal. This webinar will highlight the importance of having an integrated approach to antibody reagent development, which includes antigen design, testing, and quality control. Using examples for Western Blot, flow cytometry, and other major applications we will show some pitfalls and improvements in our 20 years of experience in developing new reagents.