This technical video provides an overview of the methodologies, tools, and reagents used to generate multicellular tumor spheroids. Specifically, this video discusses the benefits of Cultrex® extracellular matrices for spheroid formation and analysis. Technical tips are provided for using colorimetric and fluorometric assays to evaluate cell proliferation and invasion of spheroids.
This protocol video was originally recorded by Dr. Gabriel Benton at Trevigen, Inc. The Trevigen portfolio, including tools for creating 3-D and spheroid cultures, are now a part of R&D Systems, a Bio-Techne brand.
In this technical video you will learn:
- Evolution of extracellular matrices used for 3-D cell culture
- Benefits and challenges of spheroid models
- Methods for spheroid formation