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Utilizing Extracellular Matrix to Enhance Multicellular Tumor Spheroid Models for Proliferation and Invasion
Friday, September 21, 2018 - 08:47
This technical video provides an overview of the methodologies, tools, and reagents used to generate multicellular tumor spheroids. Specifically, this video discusses the benefits of Cultrex® extracellular matrices for spheroid formation and analysis. Technical tips are provided for using colorimetric and fluorometric assays to evaluate cell proliferation and invasion of spheroids.
This protocol video was originally recorded by Dr. Gabriel Benton at Trevigen, Inc. The Trevigen portfolio, including tools for creating 3-D and spheroid cultures, are now a part of R&D Systems, a Bio-Techne brand.
In this technical video you will learn:
Evolution of extracellular matrices used for 3-D cell culture
Benefits and challenges of spheroid models
Methods for spheroid formation
Tips and tricks for using Cultrex 3-D Spheroid Proliferation/Viability Assays
Preparing and evaluating Cultrex Cell Invasion Assays