Utilizing Extracellular Matrix to Enhance Multicellular Tumor Spheroid Models for Proliferation and Invasion

This technical video provides an overview of the methodologies, tools, and reagents used to generate multicellular tumor spheroids. Specifically, this video discusses the benefits of Cultrex^® extracellular matrices for spheroid formation and analysis. Technical tips are provided for using colorimetric and fluorometric assays to evaluate cell proliferation and invasion of spheroids.

This protocol video was originally recorded by Dr. Gabriel Benton at Trevigen, Inc. The Trevigen portfolio, including tools for creating 3-D and spheroid cultures, are now a part of R&D Systems, a Bio-Techne brand.

In this technical video you will learn:

• Evolution of extracellular matrices used for 3-D cell culture
• Benefits and challenges of spheroid models
• Methods for spheroid formation
• Tips and tricks for using Cultrex 3-D Spheroid Proliferation/Viability Assays
• Preparing and evaluating Cultrex Cell Invasion Assays

Related Resources:

View Spheroid Proliferation and Viability Assays

View Cultrex Cell Invasion Assays

More 3-D and Organoid Resources