Cultrex® BME Coating for Adult Stem Cell Expansion and Differentiation

This protocol video demonstrates how to surface-coat cell culture plates and transwell inserts with Cultrex^® Basement Membrane Extract, also called Cultrex^® BME. Cultrex^® BME is an extracellular matrix scaffold commonly used in stem cell culture and 3-dimensional (3-D) cell culture protocols, such as organoids.

In this protocol you will learn:

• Surface-coating protocol for Cultrex^® BME
• Optimal dilutions of Cultrex^® BME for expansion and differentiation
• General tips for working with Cultrex^® BME

Related Resources:

• Learn More about Cultrex^® Basement Membrane Extracts
• View our Organoid and 3-D Cell Culture Resource Page