Cultrex® BME Coating for Adult Stem Cell Expansion and Differentiation
This protocol video demonstrates how to surface-coat cell culture plates and transwell inserts with Cultrex® Basement Membrane Extract, also called Cultrex® BME. Cultrex® BME is an extracellular matrix scaffold commonly used in stem cell culture and 3-dimensional (3-D) cell culture protocols, such as organoids.
In this protocol you will learn:
- Surface-coating protocol for Cultrex® BME
- Optimal dilutions of Cultrex® BME for expansion and differentiation
- General tips for working with Cultrex® BME