Cultrex® BME Coating for Adult Stem Cell Expansion and Differentiation

Sunday, January 28, 2018 - 09:26

This protocol video demonstrates how to surface-coat cell culture plates and transwell inserts with Cultrex® Basement Membrane Extract, also called Cultrex® BME. Cultrex® BME is an extracellular matrix scaffold commonly used in stem cell culture and 3-dimensional (3-D) cell culture protocols, such as organoids and MimEX™ Tissue Model Systems. Using Cultrex® BME, MimEX Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3-D ex vivo organ tissue.

In this protocol you will learn:

  • Surface-coating protocol for Cultrex® BME
  • Optimal dilutions of Cultrex® BME for expansion and differentiation
  • General tips for working with Cultrex® BME

 

Related Resources:
Learn More about MimEX Tissue Model Systems
View all protocol videos for MimEX Tissue Models
Learn More about Cultrex® Basement Membrane Extracts
View our Organoid and 3-D Cell Culture Resource Page

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