Organoid and 3D Culture Reagents

Banner image showing the progression from basic cell culture to advanced organoids. Left side features a gloved hand holding a petri dish with red media, labeled 'From Simple Cell Culture'. Right side displays colorful 3D organoids with the text 'To Complex Organoids' on a blue background

Organoid and 3D Cell Culture Reagents

Organoid and three-dimensional (3D) cell culture have emerged as pivotal systems for understanding human organ development, modeling disease, screening for drug efficacy or toxicity, and investigating personalized medicine. The reagents and protocols needed for differentiation and culturing these advanced multi-cellular in vitro tissues vary by starting cell type, organ and species, and include various formulations of extracellular matrices, organoid culture media and supplements, proteins, small molecules, and antibodies.

This page serves as a reagent and technical resource to help cell biology researchers optimize culture conditions to build robust organoids with high viability. Access protocols for reproducible 3D organoid culture systems and discover new products relevant to your work in organoid research.

3D Organoid Models

 

Liver spheroids stained for CD68, Albumin, Vimentin, and DNA on the left, and DNA, panCK, and CD31 on the right following treatment with Tocris’ Tissue Clearing Pro-Organoid Kit

 

Cancer organoids should be cultured in similar conditions to those for which the parent organoid can be grown. In some instances mutations occur in cancer stem cells that allow them to grow in the absence of normal growth factors, such as EGF or FGF. Depending on the cancer organoid it may be possible to grow them in a medium where one or more factors are removed from the normal organoid growth medium.

Cancer Pathways