Organoid and three dimensional (3D) cell culture systems are emerging as pivotal models for drug screening, investigating personalized medicine, and understanding human organ development. Organoids are generated from pluripotent stem cells or organ progenitor cells that have differentiated and self-organized into a formation that resembles the structure and function of an intact mammalian organ. Use the table below as a guide to find media, matrix components, growth factors, and small molecules for generating your organoid of interest.
Cancer organoids should be cultured in similar conditions as to those for which the parent organoid can be grown. In some instances mutations occur in cancer stem cells that allow them to grow in the absence of normal growth factors, such as EGF or FGF. Depending on the cancer organoid it may be possible to grow them in a medium where one or more factors are removed from the normal organoids growth medium.