Antibodies Validated for IHC, Multiplex IHC, and Spatial Multiomics
R&D Systems IHC antibodies deliver the specificity, sensitivity, and lot-to-lot consistency you need to visualize and quantify antigen expression in tissue context across oncology, immunology, neuroscience, and more.
Precision validated monoclonal antibodies and polyclonal antibodies for FFPE tissues, multiplex panels, and spatial multiomics.
Why Choose R&D Systems for IHC?
- Quality You Can See: Unaltered IHC and Multiplex IF validated antibodies. No image enhancements.
- Multiplex-Ready: Antibody clones and formulations selected for low cross-reactivity and clean backgrounds in multiplex spatial biology experiments.
- Application Breadth: Validated for use in IHC, ICC/IF, flow cytometry, WB, and ELISA enabling cross-modality workflows.
- Formats for Any Assay: Unconjugated, enzyme-conjugated (HRP/AP), fluorophore-conjugated, biotinylated, and BSA/Azide-Free for custom labeling.
Featured Antibodies for IHC & Spatial Panels
Primary Antibody Formats for Multiplex IHC and Spatial Biology
| Category | Key Markers |
| Immune Lineage | CD3, CD4, CD8, CD11b, CD14, CD19, CD20, CD45 |
| Checkpoint / Activation | PD-1, PD-L1, LAG-3, TIM-3, ICOS |
| Tumor & Stroma | Cytokeratins, Vimentin, α-SMA, Fibronectin |
| Proliferation / Cell State | Ki67, Cleaved Caspase-3 |
| Neuro | NeuN, GFAP, Iba1, MAP2, Synaptophysin |
| Epithelial / Endothelial | E-Cadherin, N-Cadherin, CD31, VEGF |
| Format | Use Case |
| Unconjugated | Flexible, custom use; Requires Conjugated Secondary Antibodies (HRP Secondary Antibodies, HRP-Polymer Secondary Antibodies, Fluorescent Secondary Antibodies) |
| Fluorescent Conjugated | Single-plex & multiplex IF; Alexa Fluor® 488, Alexa Fluor® 647, and more |
| Biotinylated | Signal amplification |
| BSA/Azide-Free | Conjugation-Ready (metal-tag, oligo-barcode) |
| Bulk & Custom Formulation | Scale-up, custom concentrations, liquid formulations |
| Custom Antibody Development | Custom conjugation, isotype switching for multiplex, recombinant conversion |
Antibodies for Multiplex IHC, Immunofluorescence (IF) and Spatial Biology
R&D Systems polyclonal and monoclonal antibodies are used across leading spatial biology and multiomic platforms and panel types, including high-plex imaging and transcript plus protein assays. Antibodies and formulations are selected with attention to antigen preservation in FFPE, multiplex compatibility (isotype diversity, host species, and spectral separation), and low nonspecific binding to support:
- High-plex spatial imaging
- Spatial transcriptomics + protein antigen codetection workflows
- Automated stainers and open systems, including research-use imaging instruments and slide-scanners
- Platform-specific panels (e.g., Lunaphore COMET™)
IHC Validation Philosophy
Our validation approach is designed to de-risk IHC and spatial experiments. We screen IHC antibodies in multiple applications, across multiple tissue types to ensure you have the data you need to purchase your antibody with confidence.
- Specificity You Can Trust: IHC antigen results supported by orthogonal validation methods including western blot, immunofluorescence, knockout/knockdown, Dual ISH-IHC
- Tested in Diverse Biological Samples: Not limited to cell pellets, or one tissue, type, our IHC antibodies are screened in multi-tissue panels to confirm antigen engagement. Healthy and disease tissue are included when appropriate.
- Reproducibility, Lot to Lot: Our quality guarantee ensures every new lot of our antibodies are tested in validated applications. These tight QC specifications ensure consistent performance across vials and production runs. All antibodies are backed by our 100% Guarantee.
Reagents for IHC Staining
In addition to primary antibodies, R&D Systems offers a full range of complementary reagents, like secondary antibodies, staining kits, and membrane dyes, to support IHC, IF, and multiplex IHC workflows.
Resources & Support
Frequently Asked Questions:
Q: Are these antibodies suitable for FFPE samples?
A: Yes. Many R&D Systems antibodies are validated on FFPE tissues with recommended HIER conditions and dilutions.
Q: Can I use them in high-plex spatial imaging?
A: Yes. We offer carrier-free formats that are compatible with common multiplex and TSA workflows. Ask us about isotype diversity and spectral spacing.
Q: Do you provide clone-level validation data?
A: Datasheets include clone/host/isotype, immunogen, tested applications, and representative images. Additional data may be available upon request.
Q: What if I need a custom label or bulk volume?
A: We provide custom conjugation and bulk packaging; carrier-free options enable metal/DNAbarcoded labeling for spatial proteomics.
Q: How do you ensure lot-to-lot consistency?
A: Tight QC release criteria and control charts maintain performance across production lots.