14-3-3 beta/alpha Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33454

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 14-3-3 beta/alpha Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 beta/alpha. The exact sequence is proprietary.

Localization

Cytoplasm, Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 beta/alpha Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454]

Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454]

Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454]

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454]

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Rat cervix. 14-3-3 beta antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454]

Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454]

Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454]

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454]

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.
14-3-3 beta/alpha Antibody - BSA Free

Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
14-3-3 beta/alpha Antibody - BSA Free

Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for 14-3-3 beta/alpha Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 14-3-3 beta/alpha

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Alternate Names

14-3-3 alpha, 14-3-3 beta, 14-3-3 protein beta/alpha, brain protein 14-3-3, beta isoform, GW128, HS1, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, protein kinase C inhibitor protein-1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide, YWHAA

Entrez Gene IDs

7529 (Human)

Gene Symbol

YWHAB

Additional 14-3-3 beta/alpha Products

Product Documents for 14-3-3 beta/alpha Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 14-3-3 beta/alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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