ACTL6B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92909

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACTL6B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6B (NP_057272.1). MSGGVYGGDEVGALVFDIGSFSVRAGYAGEDCPKADFPTTVGLLAAEEGGGLELEGDKEKKGKIFHIDTNALHVPRDGAEVMSPLKNGMIEDWECFRAILDHTYSKHVKSEPNLHPVLMSEAPWNTRAKREKLTELMFEQYNIPAFFLCKTAVLTAFANGRSTGLVLDSGATHTTAIPVHDGYVLQQGIVKSPLAGDFISMQCRELFQEMAIDIIPPYMIAAKEPVREGAPPNWKKKEKLPQVSKSWHNYMCNEVIQDFQASVLQVSDSPYDEQVAAQMP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACTL6B Antibody - BSA Free

Western Blot: ACTL6B AntibodyBSA Free [NBP2-92909]

Western Blot: ACTL6B AntibodyBSA Free [NBP2-92909]

Western Blot: ACTL6B Antibody [NBP2-92909] - Analysis of extracts of various cell lines, using ACTL6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 40s.
Immunocytochemistry/ Immunofluorescence: ACTL6B Antibody - BSA Free [NBP2-92909]

Immunocytochemistry/ Immunofluorescence: ACTL6B Antibody - BSA Free [NBP2-92909]

Immunocytochemistry/Immunofluorescence: ACTL6B Antibody [NBP2-92909] - Analysis of A-549 cells using ACTL6B.
Immunohistochemistry-Paraffin: ACTL6B Antibody - BSA Free [NBP2-92909]

Immunohistochemistry-Paraffin: ACTL6B Antibody - BSA Free [NBP2-92909]

Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded mouse jejunum using ACTL6B.
Immunohistochemistry-Paraffin: ACTL6B Antibody - BSA Free [NBP2-92909]

Immunohistochemistry-Paraffin: ACTL6B Antibody - BSA Free [NBP2-92909]

Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded human kidney cancer using ACTL6B.
Immunohistochemistry-Paraffin: ACTL6B Antibody - BSA Free [NBP2-92909]

Immunohistochemistry-Paraffin: ACTL6B Antibody - BSA Free [NBP2-92909]

Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded human kidney using ACTL6B.

Applications for ACTL6B Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACTL6B

The protein encoded by the ACTL6B gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. (provided by RefSeq)

Alternate Names

53 kDa BRG1-associated factor B, actin-like 6, actin-like 6B, Actin-related protein Baf53b, ACTL6, ArpNalpha, BAF53Bactin-like protein 6B, BRG1-associated factor 53B, hArpN alpha

Gene Symbol

ACTL6B

Additional ACTL6B Products

Product Documents for ACTL6B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACTL6B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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