ADAR Antibody (CL0176) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57891

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL0176

Format

Azide and BSA Free
View all ADAR Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P55265, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ADAR Antibody (CL0176) - Azide and BSA Free

ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Analysis in human cell line U-251MG.
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-57826 antibody. Corresponding ADAR RNA-seq data are presented for the same tissues.
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADAR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Staining of A549 cells using the Anti-ADAR monoclonal antibody, showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Staining of A549 cells using the Anti-ADAR monoclonal antibody, showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Staining of human kidney shows strong nuclear positivity in cells in tubules.
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Staining of human rectum shows strong nuclear positivity in glandular cells.
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Staining of human skeletal muscle shows no positivity in myocytes as expected.
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
ADAR Antibody (CL0176) - BSA Free

ADAR Antibody (CL0176) - BSA Free

Staining of human tonsil shows strong nuclear positivity in lymphoid cells.

Applications for ADAR Antibody (CL0176) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ADAR

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Double-stranded RNA-specific adenosine deaminase

Alternate Names

ADAR1, DRADA, DSRAD, EC 3.5.4.37, G1P1, IFI-4, IFI4, K88DSRBP, p136

Gene Symbol

ADAR

Additional ADAR Products

Product Documents for ADAR Antibody (CL0176) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ADAR Antibody (CL0176) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ADAR Antibody (CL0176) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review ADAR Antibody (CL0176) - Azide and BSA Free and earn rewards!

Have you used ADAR Antibody (CL0176) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies