AKR1A1 Antibody (24GB6220)

Novus Biologicals | Catalog # NBP3-51748

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 24GB6220
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Product Specifications

Immunogen

Recombinant protein of human AKR1A1 (Uniprot: P14550)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Scientific Data Images for AKR1A1 Antibody (24GB6220)

AKR1A1 Antibody (24GB6220)

Immunocytochemistry/ Immunofluorescence: AKR1A1 Antibody (24GB6220)[NBP3-51748] -

Immunocytochemical staining of HepG2 cells with AKR1A1 antibody (1:1,000). Nuclei were stained blue with DAPI; AKR1A1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 um.
AKR1A1 Antibody (24GB6220)

Flow Cytometry: AKR1A1 Antibody (24GB6220)[NBP3-51748] -

Validation of AKR1A1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with AKR1A1 antibody (1:2,000) and analyzed using BD flow cytometer.
AKR1A1 Antibody (24GB6220)

Flow Cytometry: AKR1A1 Antibody (24GB6220)[NBP3-51748] -

Flow cytometric analysis of AKR1A1expression in HepG2 cells using AKR1A1 antibody (1:2,000). Green, isotype control; red, AKR1A1.

Applications for AKR1A1 Antibody (24GB6220)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AKR1A1

AKR1A1 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Alternative splicing of this gene results in two transcript variants encoding the same protein.

Long Name

Aldo-keto reductase family 1 member A1

Alternate Names

Aldehyde reductase, ALDR1, EC 1.1.1.19, EC 1.1.1.2, EC 1.1.1.20, EC 1.1.1.372, EC 1.1.1.54

Gene Symbol

AKR1A1

Additional AKR1A1 Products

Product Documents for AKR1A1 Antibody (24GB6220)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for AKR1A1 Antibody (24GB6220)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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