alcohol dehydrogenase 5 Antibody (24GB4695)

Novus Biologicals | Catalog # NBP3-50809

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4695
View all alcohol dehydrogenase 5 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human alcohol dehydrogenase 5 (Uniprot: P11766)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alcohol dehydrogenase 5 Antibody (24GB4695)

alcohol dehydrogenase 5 Antibody (24GB4695)

Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody (24GB4695)[NBP3-50809] -

Immunocytochemical staining of HepG2 cells with alcohol dehydrogenase 5 antibody (1:1,000). Nuclei were stained blue with DAPI; alcohol dehydrogenase 5 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
alcohol dehydrogenase 5 Antibody (24GB4695)

Flow Cytometry: alcohol dehydrogenase 5 Antibody (24GB4695)[NBP3-50809] -

Flow cytometric analysis of alcohol dehydrogenase 5expression in HepG2 cells using alcohol dehydrogenase 5 antibody (1:2,000). Green, isotype control; red, ADH5.
alcohol dehydrogenase 5 Antibody (24GB4695)

Western Blot: alcohol dehydrogenase 5 Antibody (24GB4695)[NBP3-50809] -

Western blotting analysis using alcohol dehydrogenase 5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for alcohol dehydrogenase 5 Antibody (24GB4695)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 5

The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.

Alternate Names

ADH-3, ADHXEC 1.1.1.1, alcohol dehydrogenase (class III), chi polypeptide, Alcohol dehydrogenase 5, alcohol dehydrogenase 5 (class III), chi polypeptide, Alcohol dehydrogenase class chi chain, alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, EC 1.1.1, EC 1.1.1.-, EC 1.1.1.284, FALDH, FDHGSNOR, formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, S-(hydroxymethyl)glutathione dehydrogenase

Gene Symbol

ADH5

Additional alcohol dehydrogenase 5 Products

Product Documents for alcohol dehydrogenase 5 Antibody (24GB4695)

Certificate of Analysis

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Download SDS

Product Specific Notices for alcohol dehydrogenase 5 Antibody (24GB4695)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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