alpha Tubulin 4a Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-21604

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, C. elegans, Chicken, Drosophila, Insect, Protozoa

Predicted:

Bovine (100%), Chimpanzee (99%), Sheep (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alpha Tubulin 4a Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary.

Reactivity Notes

Mosquito (100%), Xenopus laevis (92%).

Localization

Cytoplasm; Cytoskeleton; Microtubule

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Tubulin 4a Antibody - BSA Free

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604]

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604]

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604]

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604]

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 10% SDS PAGE gel.
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604]

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604]

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604]

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604]

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - Rat esophagus. alpha Tubulin 4a stained by alpha Tubulin 4a antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
alpha Tubulin 4a Antibody

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] -

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Tubulin 4a Antibody

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] -

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Tubulin 4a Antibody

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] -

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Tubulin 4a Antibody

Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP2-21604] -

Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.
Blue: Fluoroshield with DAPI.
alpha Tubulin 4a Antibody - BSA Free

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] -

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.
A. 30 ug Drosophila lysate/extract
10% SDS-PAGE
alpha Tubulin antibody (NBP2-21604) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha Tubulin 4a Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP2-21604] -

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (NBP2-21604) diluted at 1:500.
Blue: Fluoroshield with DAPI.
alpha Tubulin 4a Antibody - BSA Free

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] -

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (NBP2-21604) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha Tubulin 4a Antibody - BSA Free

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (NBP2-21604) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha Tubulin 4a Antibody - BSA Free

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (NBP2-21604) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for alpha Tubulin 4a Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha Tubulin 4a

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq]

Long Name

Tubulin alpha-4A chain

Alternate Names

Alpha-tubulin 1, TUBA1, TUBA4A, Tubulin H2-alpha

Gene Symbol

TUBA4A

Additional alpha Tubulin 4a Products

Product Documents for alpha Tubulin 4a Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for alpha Tubulin 4a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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