AlphaB Crystallin/CRYAB Antibody (24GB6070)

Novus Biologicals | Catalog # NBP3-51002

Novus Biologicals
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB6070
View all AlphaB Crystallin/CRYAB Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human AlphaB Crystallin/CRYAB (Uniprot: P02511)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (24GB6070)

AlphaB Crystallin/CRYAB Antibody (24GB6070)

Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (24GB6070)[NBP3-51002] -

Immunocytochemical staining of HT-1080 cells with AlphaB Crystallin/CRYAB antibody (1:1,000). Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
AlphaB Crystallin/CRYAB Antibody (24GB6070)

Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (24GB6070)[NBP3-51002] -

Flow cytometric analysis of AlphaB Crystallin/CRYABexpression in H9c2 cells using crystallin alpha B antibody (1:2,000). Green, isotype control; red, crystallin alpha B.
AlphaB Crystallin/CRYAB Antibody (24GB6070)

Western Blot: AlphaB Crystallin/CRYAB Antibody (24GB6070)[NBP3-51002] -

Western blotting analysis using AlphaB Crystallin/CRYAB antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
AlphaB Crystallin/CRYAB Antibody (24GB6070)

Western Blot: AlphaB Crystallin/CRYAB Antibody (24GB6070)[NBP3-51002] -

Western blotting analysis using AlphaB Crystallin/CRYAB antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for AlphaB Crystallin/CRYAB Antibody (24GB6070)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Gene Symbol

CRYAB

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB Antibody (24GB6070)

Certificate of Analysis

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Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (24GB6070)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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