![Western Blot: ANKRD28 Antibody [NB100-41419]](https://resources.rndsystems.com/images/products/ANKRD28-Antibody-Western-Blot-NB100-41419-img0003.jpg "Western Blot: ANKRD28 Antibody [NB100-41419]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 975 and 1025 of human ankyrin repeat domain 28 (phosphatase interactor targeting K protein) using the numbering given in entry NP_056014.2 (GeneID 23243).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ANKRD28 Antibody
Western Blot: ANKRD28 Antibody [NB100-41419]
Western Blot: ANKRD28 Antibody [NB100-41419] - Whole cell lysate from HeLa cells (5, 15 and 50 mcg for WB; 1 mg for IP, 20 percent of IP loaded) and 293T (T; 50 mcg) cells. NB100-41419 used for WB at 0.02 mug/ml (A) and 1 mug/ml (B) and used for IP at 3 mcg/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody NB100-41420, which recognizes a downstream epitope.![Immunohistochemistry: ANKRD28 Antibody [NB100-41419]](https://resources.rndsystems.com/images/products/ANKRD28-Antibody-Immunohistochemistry-NB100-41419-img0005.jpg "Immunohistochemistry: ANKRD28 Antibody [NB100-41419]")
Immunohistochemistry: ANKRD28 Antibody [NB100-41419]
Immunohistochemistry: ANKRD28 Antibody [NB100-41419] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ANKRD28/PITK used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.![Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419]](https://resources.rndsystems.com/images/products/ANKRD28-Antibody-Immunohistochemistry-Paraffin-NB100-41419-img0001.jpg "Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419]")
Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419]
Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419] - FFPE section of human stomach carcinoma. Antibody used at a dilution of 1:250.Applications for ANKRD28 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ANKRD28
Alternate Names
ankyrin repeat domain 28, Ankyrin repeat domain-containing protein 28, KIAA0379PITKPP6-ARS-Aphosphatase interactor targeting K protein, Phosphatase interactor targeting protein hnRNP K, protein phosphatase 6 ankyrin repeat subunit A, serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A, Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A
Entrez Gene IDs
23243 (Human)
Gene Symbol
ANKRD28
UniProt
Additional ANKRD28 Products
Product Documents for ANKRD28 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ANKRD28 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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