Apolipoprotein F Antibody

Novus Biologicals | Catalog # NBP1-20882

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-KSYDLDPGAGSLE corresponding to C-Terminus according to NP_001629.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apolipoprotein F Antibody

Western Blot: Apolipoprotein F Antibody [NBP1-20882]

Western Blot: Apolipoprotein F Antibody [NBP1-20882]

Western Blot: Apolipoprotein F Antibody [NBP1-20882] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882]

Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882]

Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Apolipoprotein F Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human liver lysates (calculated MW of 35.4 kDa band according to NP_001629.1). IHC-P: Human liver shows staniing of droplets in the cytoplasm of hepatocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apolipoprotein F

The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol.

Alternate Names

Apo-F, apolipoprotein F, DKFZp781G18150, Lipid transfer inhibitor protein, LTIP, MGC22520

Entrez Gene IDs

319 (Human)

Gene Symbol

APOF

UniProt

Additional Apolipoprotein F Products

Product Documents for Apolipoprotein F Antibody

Certificate of Analysis

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Product Specific Notices for Apolipoprotein F Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Apolipoprotein F Antibody

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Protocols

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