Aquaporin-4 Antibody (CL0178)

Novus Biologicals | Catalog # NBP3-52443

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL0178
View all Aquaporin 4/AQP4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P55087, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Aquaporin-4 Antibody (CL0178)

Aquaporin-4 Antibody (CL0178) Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-52439]

Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-52439]

Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.
Aquaporin-4 Antibody (CL0178) Immunohistochemistry-Paraffin: YAP1 Antibody (CL13628) [NBP3-52440]

Immunohistochemistry-Paraffin: YAP1 Antibody (CL13628) [NBP3-52440]

Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.
Aquaporin-4 Antibody (CL0178) Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-52439]

Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-52439]

Staining of human liver shows no positivity in hepatocytes as expected.
Aquaporin-4 Antibody (CL0178) Immunohistochemistry-Paraffin: YAP1 Antibody (CL13628) [NBP3-52440]

Immunohistochemistry-Paraffin: YAP1 Antibody (CL13628) [NBP3-52440]

Staining of human stomach shows moderate to strong membranous positivity in glandular cells.
Aquaporin-4 Antibody (CL0178) Immunohistochemistry-Paraffin: Syntaxin 7 Antibody (CL0257) [NBP3-52445]

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody (CL0257) [NBP3-52445]

Analysis in human cerebral cortex and liver tissues. Corresponding RNA-seq data are presented for the same tissues.
Aquaporin-4 Antibody (CL0178) Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP3-52446]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP3-52446]

Analysis in human cerebral cortex tissue.

Applications for Aquaporin-4 Antibody (CL0178)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aquaporin 4/AQP4

The Aquaporin-4 gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selectivechannels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Twoalternatively spliced tra

Long Name

Aquaporin 4

Alternate Names

AQP-4, AQP4, MIWC, WCH4

Gene Symbol

AQP4

Additional Aquaporin 4/AQP4 Products

Product Documents for Aquaporin-4 Antibody (CL0178)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Aquaporin-4 Antibody (CL0178)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

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Protocols

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FAQs

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