ARFGAP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38787

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ARFGAP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKASEL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARFGAP1 Antibody - BSA Free

Western Blot: ARFGAP1 Antibody [NBP2-38787]

Western Blot: ARFGAP1 Antibody [NBP2-38787]

Western Blot: ARFGAP1 Antibody [NBP2-38787] - Analysis using Anti-ARFGAP1 antibody NBP2-38787 (A) shows similar pattern to independent antibody NBP2-14305 (B).
Immunocytochemistry/ Immunofluorescence: ARFGAP1 Antibody [NBP2-38787]

Immunocytochemistry/ Immunofluorescence: ARFGAP1 Antibody [NBP2-38787]

Immunocytochemistry/Immunofluorescence: ARFGAP1 Antibody [NBP2-38787] - Staining of human cell line CACO-2 shows localization to cytosol, the Golgi apparatus & vesicles. Antibody stainign is shown in green.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787]

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787]

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787]

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787]

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787] - Staining of human pancreas shows strong cytoplasm granular positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787]

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787]

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787]

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787]

Immunohistochemistry-Paraffin: ARFGAP1 Antibody [NBP2-38787] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for ARFGAP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARFGAP1

ARFGAP1 is encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

ADP-ribosylation factor GTPase-activating protein 1

Alternate Names

ARF GAP 1, ARF1 GAP, ARF1GAP

Gene Symbol

ARFGAP1

UniProt

Q8N6T3 (Human)

Additional ARFGAP1 Products

Product Documents for ARFGAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ARFGAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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