ARHGDIG Antibody

Name: ARHGDIG Antibody
Brand: Novus Biologicals
SKU: NBP1-49854
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-49854

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-EAVPEYRAPGRK corresponding to C-Terminus according to NP_001167.2.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARHGDIG Antibody

Western Blot: ARHGDIG Antibody [NBP1-49854]

Western Blot: ARHGDIG Antibody [NBP1-49854] - Western blot analysis of ARHGDIG in HeLa lysate (35ug protein in RIPA buffer) using NBP1-49854 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ARHGDIG Antibody [NBP1-49854]

Immunohistochemistry-Paraffin: ARHGDIG Antibody [NBP1-49854] - 3.75ug/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Applications for ARHGDIG Antibody

Application

Recommended Usage

Immunohistochemistry

0.5-2ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.5-2 ug/ml

Application Notes

Western blot: Approx 25kDa band observed in lysates of cell line HeLa (calculated MW of 25.1kDa according to NP_001167.2). Primary incubation was 1 hour
IHC: Paraffin embedded Human Brain (Cortex).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARHGDIG

ARHGDIG inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms ofpost-translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammaliancells. Stimulates the release of the

Alternate Names

Rho GDI 3, Rho GDP dissociation inhibitor (GDI) gamma, rho GDP-dissociation inhibitor 3, RhoGDI gamma, Rho-GDI gamma, RHOGDI-3

Entrez Gene IDs

398 (Human); 14570 (Mouse); 360500 (Rat)

Gene Symbol

ARHGDIG

UniProt

Q99819 (Human)

Additional ARHGDIG Products

Product Documents for ARHGDIG Antibody

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Certificate of Analysis

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Product Specific Notices for ARHGDIG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARHGDIG Antibody

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