ARID1A Antibody (23GB3230)

Novus Biologicals | Catalog # NBP3-51208

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3230
View all ARID1A Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ARID1A (Uniprot: O14497)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARID1A Antibody (23GB3230)

ARID1A Antibody (23GB3230)

Immunocytochemistry/ Immunofluorescence: ARID1A Antibody (23GB3230)[NBP3-51208] -

ARID1A antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: ARID1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
ARID1A Antibody (23GB3230)

Immunocytochemistry/ Immunofluorescence: ARID1A Antibody (23GB3230)[NBP3-51208] -

Immunocytochemical staining of C2C12 cells with ARID1A (1:1,000). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
ARID1A Antibody (23GB3230)

Flow Cytometry: ARID1A Antibody (23GB3230)[NBP3-51208] -

Validation of ARID1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ARID1A antibody (1:2,000) and analyzed using BD flow cytometer.
ARID1A Antibody (23GB3230)

Flow Cytometry: ARID1A Antibody (23GB3230)[NBP3-51208] -

Flow cytometric analysis of ARID1Aexpression in C2C12 cells using ARID1A antibody (1:2,000). Green, isotype control; red, ARID1A.
ARID1A Antibody (23GB3230)

Western Blot: ARID1A Antibody (23GB3230)[NBP3-51208] -

Western blotting analysis using ARID1A antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for ARID1A Antibody (23GB3230)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARID1A

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250A, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1

Gene Symbol

ARID1A

Additional ARID1A Products

Product Documents for ARID1A Antibody (23GB3230)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ARID1A Antibody (23GB3230)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ARID1A Antibody (23GB3230)

There are currently no reviews for this product. Be the first to review ARID1A Antibody (23GB3230) and earn rewards!

Have you used ARID1A Antibody (23GB3230)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies