ARPC1A Antibody

Novus Biologicals | Catalog # NBP2-11579

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ARPC1A Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DFKTLESSIQGLR corresponding to C-Terminus according to NP_001177925.1.

Epitope

CDFKTLESSIQGLR

Specificity

This antibody is expected to recognize both reported isoforms (NP_006400.2; NP_001177925.1). No cross-reactiviry expected with ARPC1B.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ARPC1A Antibody

Western Blot: ARPC1A Antibody [NBP2-11579]

Western Blot: ARPC1A Antibody [NBP2-11579]

Western Blot: ARPC1A Antibody [NBP2-11579] - Staining of fetal Mouse Brain (A) and adult Rat Brain (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ARPC1A Antibody [NBP2-11579]

Immunohistochemistry-Paraffin: ARPC1A Antibody [NBP2-11579]

Immunohistochemistry-Paraffin: ARPC1A Antibody [NBP2-11579] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: ARPC1A Antibody [NBP2-11579]

Western Blot: ARPC1A Antibody [NBP2-11579]

Western Blot: ARPC1A Antibody [NBP2-11579] (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ARPC1A Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in HeLa lysates (calculated MW of 41.6 kDa band according to NP_006400.2). Approx. 37 kDa band observed in fetal mouse brain and adult rat brain lysates (calculated MW of 41.6 kDa band according to mouse NP_062741.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARPC1A

The ARPC1A gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they bo

Alternate Names

actin binding protein (Schizosaccharomyces pombe sop2-like), actin related protein 2/3 complex, subunit 1A (41 kD), actin related protein 2/3 complex, subunit 1A, 41kDa, actin-related protein 2/3 complex subunit 1A, SOP2Hs, SOP2LArc40, SOP2-like protein

Entrez Gene IDs

10552 (Human); 56443 (Mouse); 81824 (Rat)

Gene Symbol

ARPC1A

Additional ARPC1A Products

Product Documents for ARPC1A Antibody

Certificate of Analysis

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Product Specific Notices for ARPC1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARPC1A Antibody

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Protocols

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