ATPB Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90816

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATPB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVD

Reactivity Notes

Reactivity reported in scientific literature (PMID: 21232647)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATPB Antibody - BSA Free

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90816 (A) shows similar protein distribution across tissues to independent antibody NBP1-90815 (B).
Western Blot: ATPB Antibody [NBP1-90816]

Western Blot: ATPB Antibody [NBP1-90816]

Western Blot: ATPB Antibody [NBP1-90816] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATPB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate immunoreactivity in glomeruli.
Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816]

Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
ATPB Antibody - BSA Free Immunohistochemistry: ATPB Antibody - BSA Free [NBP1-90816]

Immunohistochemistry: ATPB Antibody - BSA Free [NBP1-90816]

Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
ATPB Antibody - BSA Free Immunohistochemistry: ATPB Antibody - BSA Free [NBP1-90816]

Immunohistochemistry: ATPB Antibody - BSA Free [NBP1-90816]

Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.
ATPB Antibody - BSA Free Western Blot: ATPB Antibody - BSA Free [NBP1-90816]

Western Blot: ATPB Antibody - BSA Free [NBP1-90816]

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
ATPB Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: ATPB Antibody [NBP1-90816]

Immunocytochemistry/ Immunofluorescence: ATPB Antibody [NBP1-90816]

Staining of human cell line A-549 using Anti-ATP5B antibody HPA001520, showing protein localization to Mitochondria (green). Microtubule and nuclear probes are visualized in red and blue, respectively (where available).

Applications for ATPB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATPB

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

Alternate Names

ATP synthase subunit beta, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, ATPMB, ATPSBmitochondrial ATP synthase beta subunit, EC 3.6.3, EC 3.6.3.14, MGC5231, mitochondrial ATP synthetase, beta subunit

Gene Symbol

ATP5F1B

Additional ATPB Products

Product Documents for ATPB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ATPB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATPB Antibody - BSA Free

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Protocols

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FAQs

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