Bax Antibody (6A7) - Azide and BSA Free

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• (5)

• Bax Antibody (6A7) - Azide and BSA Free

  Name: Arpit Kumar

  Application: Western Blot

  Sample Tested: IHC on parrafin sections (mouse) and IHC Sample Tested

  Species: Human, Mouse and Rat

  Verified Customer | Posted 06/17/2026

  Stained sections were observed with a light microscope coupled with a digitally calibrated camera at 10- and 40-fold magnifications. BAX immunoreactivity was graded according to the intensity in NFL, GCL, IPL, INL, OPL, ONL, IS/OS. We performed a semi quantitative scoring method with a typical H-scoring system; which represents staining intensity (0 = no staining; 1 = weak; 2 = moderate; 3 = strong) by the percentage of positive cells in sections. Scores ranged from 0 to 300 (stained intensity multiplied by the percentage of stained cells in percent). Images were quantitatively analyzed for DAB optical density values with ImageJ software in combination with the color deconvolution plug-in in order to differentiate the signals for DAB from hematoxylin. The scale bar of 50 m was applied. Negative control incubations were performed for every experiment, where the primary antibody was replaced by a matching mouse IgG of the same isotype at an equivalent concentration, since none was observed in negative control experiments (in the absence of primary antibody with equivalent concentration matched to mouse IgG isotype negative controls), the staining with BAX signal specificity was thus validated.

  

• Bax Antibody (6A7)

  Name: Sweta Ghosh

  Application: Western Blot

  Sample Tested: Cell lysate from colon cancer cell line and Melanoma cells

  Species: Mouse and Human

  Verified Customer | Posted 04/19/2024

  Bax western blot in cancer cell lysate after apoptosis induction

  

• Bax Antibody (6A7)

  Name: Hayk Simonyan

  Application: Western Blot

  Sample Tested: mouse liver

  Species: Mouse

  Verified Customer | Posted 06/23/2017

  1:1000, liver lysate 10mg

  

• Name: Anonymous

  Application: Western Blot

  Sample Tested:

  Species: Human

  Verified Customer | Posted 03/31/2014

  Bax western blot with Pancreatic cancer cell line L3.6pl.

  

• Name: Anonymous

  Application: Immunohistochemistry-Frozen

  Sample Tested: mouse retina

  Species: Mouse

  Verified Customer | Posted 07/28/2012

  

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