Bcl-xL Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56104

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Mouse, Rat, Canine

Predicted:

Bovine (100%), Gerbil (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bcl-xL Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 46-66 (ETPSAINGNPSWHLADSPAVN) of human Bcl-xL was used as immunogen; GenBank no. CAA80661.1. The immunogen sequence is 100% conserved in Bcl-XS. The immunogen sequence is 100% in multiple species including mouse, rat, dog, cow, gerbil, and sheep.

Reactivity Notes

Mouse, Canine, Rat reactivity reported in scientific literature (PMID: 15380478).

Specificity

Bcl-XL+S

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl-xL Antibody - BSA Free

Western Blot: Bcl-xL Antibody [NB100-56104]

Western Blot: Bcl-xL Antibody [NB100-56104]

Western Blot: Bcl-xL Antibody [NB100-56104] - WB analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is detected. Lane 8. Recombinant Bcl-XL (positive control).
Western Blot: Bcl-xL Antibody [NB100-56104]

Western Blot: Bcl-xL Antibody [NB100-56104]

Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104]

Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104]

Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-xL expression. The bottom image was negative for Bcl-xL expression. The adjacent stratum spinosum and middle stratum granulosum layers were both very positive for Bcl-xL expression. The top stratum corneum consisting mainly of dead or peeling cells were both negative for Bcl-xL expression.
Western Blot: Bcl-xL Antibody [NB100-56104]

Western Blot: Bcl-xL Antibody [NB100-56104]

Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in lysates from isolated rat liver mitochondria using this antibody. The antibody detected Bcl-xL at ~30 kDa.
Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104]

Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104]

Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in human breast carcinoma at 1:2000. Formalin-fixed, paraffin-embedded tissue section from invasive ductal carcinoma. An organellar/mitochondrial pattern of Bcl-xL staining is observed in the cytosol of tumor cells.
Bcl-xL Antibody - BSA Free

Western Blot: Bcl-xL Antibody - BSA Free [NB100-56104] -

Nuclear translocation of TCA cycle enzymes prevents doxorubicin-mediated cellular damage.a–c Enzyme activity of IDH-2 (n = 4) (a), MDH-2 (n = 3) (b), and PDHC (n = 3) (c) in nuclei isolated from cells transduced with tetracycline-inducible NLS-IDH-2 constructs. Activity is represented as the rate in nM/min or delta OD/min, p value* <0.05, p value**** = 0.0001, and lysates were probed for PCNA to detect equal nuclear protein amounts used for the assay. d Reduced apoptotic cell death as measured by Annexin:V:FITC staining in cells expressing nuclear TCA cycle dehydrogenases prior to doxorubicin DRN treatment. N = 3 p value* <0.05 (e) Quantification of troponin I released from cardiomyocytes as a marker of cardiac injury, minimum n = 4 p value** <0.007, p value* <0.05. f Cell death as measured by Cell Titer GloTM in cells expressing nuclear dehydrogenases prior to DRN treatment. minimum n = 4 p value*** = 0.0001, p value**** <0.0001. NLS-EGFP-2A-NLS-mCherry was used as a control for the 2 A linker system. g DNA damage as assessed by ɣ-H2AX foci formation. Scale bar 20 um. h, i qRT‒PCR showing the expression of anti-apoptotic genes BCL2 (h) and MCL1 (i). n = 3 P value ** <0.005, *<0.05. j–l Protein levels of anti-apoptotic proteins (j, k along with reduced caspase 9 cleavage (l) in cells expressing nuclear IDH-2 compared to cells treated with doxorubicin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37468519), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Bcl-xL Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)

Reviewed Applications

Read 1 review rated 4 using NB100-56104 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-xL

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. NB100-56104 recognizes Bcl-xL and Bcl-xS. Human Bcl-xL is a 233 amino acid protein; GenBank no. CAA80661.1. Human Bcl-xS is a 170 amino acid protein; GenBank no. CAA80662.1.

Long Name

B Cell Lymphoma/Leukemia x Long Form

Alternate Names

BclxL

Entrez Gene IDs

598 (Human); 12048 (Mouse); 24888 (Rat)

Gene Symbol

BCL2L1

Additional Bcl-xL Products

Product Documents for Bcl-xL Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Bcl-xL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bcl-xL Antibody - BSA Free

Customer Reviews for Bcl-xL Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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4 Stars
100%
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Daoy whole cell lysate (25ug)
    Species: Human
    Verified Customer | Posted 07/15/2016
    Western blot analysis of Bclxl in Daoy cell lysate (25ug)
    Bcl-xL Antibody - BSA Free NB100-56104

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